![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K642001:Design
Designed by: Wilson Lam, Matthew Orton Group: iGEM11_uOttawa (2011-09-22)
LacI repressor tagged with yBFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1084
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
LacI was designed without a stop codon to enable the construction of a fusion protein with yeast codon optimized BFP. BFP was also designed without a stop codon for the purposes of integrating into the Ade2 locus of S. cerevisiae. This Ade2 locus contains a terminator region flanking BFP downstream.
Source
LacI was PCR amplified from a part that was submitted last year by the uOttawa team (BBa_K319007). Yeast codon optimized BFP was synthesized by the uOttawa team using Mr. Gene DNA synthesis.